Composite

Part:BBa_K2637042:Design

Designed by: Jinyu Yang   Group: iGEM18_Tianjin   (2018-10-05)


Gal1 promoter+mCherry+ADH1 terminator+Gal2 promoter+NanoLuc+CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2890
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2098


Design Notes

No.


Source

We copied the Gal2 promoter, CYC1 terminator and ADH1T terminator sequences from plasmid BY4741 and utilized the part numbered BBa_E2060 to obtain the sequence of mCherry and the part numbered BBa_K1680009 to gain the sequence of NanoLuc. As for the sequence of Gal1, it is the work from TU Munich's part.

References